Fayl:Phage display.png
Bu prevyuning hajmi: 800 × 509 piksel. Boshqa oʻlchamlari: 320 × 204 piksel | 640 × 407 piksel | 1 024 × 652 piksel | 1 280 × 814 piksel | 2 826 × 1 798 piksel.
Asl fayl (2 826 × 1 798 piksel, fayl hajmi: 612 KB, MIME tipi: image/png)
Qisqa izoh
| Taʼrif |
English: Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated. 5) those genes may have random mutations introduced and used to perform another round of evolution. The selection and amplification steps can be performed multiple times at greater stringency to isolate higher-affinity binders.
Français : traduction à améliorer : Cycle d'évolution. 1) Protéines de fusion pour une protéine virale d'enveloppe + le gène devant évoluer (typiquement un fragment d'anticorps) sont exprimées dans le bactériophage. 2) La banque de phages est lavées sur une cible immobilisée. 3) les liants à haute affinité restants sont utilisés pour infecter les bactéries. 4) les gènes codant pour les liants à haute affinité sont isolés. 5) ces gènes peuvent provoquer des mutations aléatoires introduites et utilisées pour effectuer un autre cycle d'évolution. Les étapes de sélection et d’amplification peuvent être effectuées plusieurs fois avec une plus grande rigueur pour isoler des liants d’affinité plus élevée. |
| Sanasi | |
| Manba | Oʻzimning ishim |
| Muallif | Thomas Shafee |
Litsenziyalash
I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution 4.0 International license.
- Siz erkinsiz:
- ulashishga – ishlanmani nusxalash, tarqatish va uzatish
- remiks qilishga – ishni moslashtirishga
- Quyidagi shartlar asosida:
- atribut – Siz tegishli litsenziyaga havolani taqdim etishingiz va oʻzgartirishlar kiritilganligini koʻrsatishingiz kerak. Siz buni har qanday oqilona yoʻl bilan qilishingiz mumkin, lekin litsenziar Sizni yoki Sizning foydalanishingizni ma'qullashini taklif qiladigan tarzda emas.
Fayl tarixi
Faylning biror paytdagi holatini koʻrish uchun tegishli sana/vaqtga bosingiz.
| Sana/Vaqt | Miniatura | Oʻlchamlari | Foydalanuvchi | Izoh | |
|---|---|---|---|---|---|
| joriy | 07:53, 22-May 2019 | | 2 826 × 1 798 (612 KB) | Evolution and evolvability | minor optimisation of label positions |
| 05:29, 22-May 2019 |  | 2 901 × 1 798 (585 KB) | Evolution and evolvability | User created page with UploadWizard |
Fayllarga ishoratlar
Bu faylga bogʻlangan sahifalar yoʻq.
Faylning global foydalanilishi
Ushbu fayl quyidagi vikilarda ishlatilyapti:
- da.wikipedia.org loyihasida foydalanilishi
- en.wikipedia.org loyihasida foydalanilishi
- fa.wikipedia.org loyihasida foydalanilishi
- sv.wikipedia.org loyihasida foydalanilishi
- tr.wikipedia.org loyihasida foydalanilishi
- www.wikidata.org loyihasida foydalanilishi
- zh.wikipedia.org loyihasida foydalanilishi
